NSLS-II   |   CFN   |   Brookhaven National Laboratory

Workshop 4: Macromolecular Serial Crystallography using the LCP Jet Injector

Date: Monday, May 20, 2019

Organizer(s): Jose Martin-Garcia (Arizona State University); Martin Fuchs (NSLS-II/BNL), Wuxian Shi (NSLS-II/BNL)

Location: Physics Large Seminar Room, Bldg 510

Description:

Since the first successful serial crystallography experiment at a synchrotron radiation source, the popularity of this approach has continued to grow, showing that current micro-focus beamlines at 3rd generation synchrotrons can be viable alternatives to scarce X-ray free electron laser sources. To date, the LCP jet sample delivery has been demonstrated to be suitable for determining numerous crystallographic structures from micro-crystals of membrane proteins grown in LCP medium as well as from previously grown crystals of soluble proteins. One of the major advantages of using the high-viscosity injector at FELs is the reduction in sample consumption compared to liquid injectors (or GDVN). At storage rings, the slower flow speeds of high-viscosity injectors are the basis of extending the passage time of crystals through the X-ray beam sufficiently to obtain usable diffraction. A main appeal here is the possibility to deliver LCP grown crystals directly to the beam with minimal disturbance.

Through a Partner User collaboration between the team from Arizona State University around John Spence and NSLS-II, serial crystallography data collection with a viscous jet is currently being established at the FMX (17-ID-2) beamline. The goal of this 1-day workshop is to reach out to the NSLS-II structural biology user community by providing an overview of the technical aspects and of the scientific applications and opportunities of serial crystallography with high viscosity injectors. A practical session at the FMX beamline will be included as part of the workshop to enable further discussion and familiarize the users with the requirements for using the technique.

StartsEndsList of Speakers
9:00 AM9:20 AM Welcome Remarks, Organizers
(Continental Breakfast - included)
9:20 AM9:50 AM John Spence, Arizona State University
Serial Microcrystallography - Sucessess, Opportunities and Problems
9:50 AM10:20 AM Jose Martin-Garcia, Arizona State University
Preparation and Characterization of Micro-crystals for Serial Crystallography
10:20 AM10:40 AM Coffee Break, (provided)
All
10:40 AM11:10 AM Uwe Weierstall, Arizona State University
Serial Crystallography with a High Viscosity Injector
11:10 AM11:20 AM Chufeng Li, Arizona State University
Serial Crystallography Data Collection and Analysis
11:20 AM11:40 AM Sabine Botha, Arizona State University
In situ LCP Crystal Derivatization and SAD Phase Retrieval for Serial
11:40 AM12:10 PM Ti-Yen Lan, Princeton University
Solving Protein Structure from Sparse Serial Microcrystallography Data Collected at a Storage Ring Source
12:10 PM1:30 PM Lunch Break, (provided)
All
1:30 PM2:00 PM Vadim Cherezov, University of Southern California
Serial Crystallography of GPCRs at Synchrotrons and XFELs
2:00 PM2:30 PM Joerg Standfuss, Paul Scherrer Institute
Dynamics of Bacteriorhodopsin Activation Studied at Synchrotrons and X-ray Lasers
2:30 PM2:50 PM Coffee Break, (provided)
Group Photo
2:50 PM5:50 PM
Practical Demonstration in the Soft & Bio Materials Wet Lab (1L-L07) and in the Structural Biology Wet Lab (1L-L08) (sample preparation) and at Beamline 17-ID-2 (injection and data processing)
5:50 PM6:20 PM Discussion and Closing Remarks, Workshop Organizers
All
6:20 PM  Workshop Adjourns, Workshop Organizers
Thank You for Your Participation